Prepared Plated Media PPM

BLOOD AGAR BASE

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Catalog Number : 912

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Description

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BLOOD AGAR BASE is used for the isolation, cultivation and detection of hemolytic reaction of fastidious microorganisms. It is suitable for isolating and cultivating a wide range of microorganisms with diffi cult growth characteristics. Upon adding blood, it can be utilized for determining hemolytic reactions.  The Heart infusion and Meat peptone are rich sources of nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bacteriological agar is the solidifying agent. The addition of blood provides extra growth factors for fastidious microorganisms and is the basis for determining hemolytic reactions. Hemolytic patterns may vary with the type of blood or base medium used. For example, defi brinated sheep blood allows the recovery of Thermophilus species and gives best results for Group A  treptococci. Use standard procedures to obtain isolated colonies from specimens. Incubate at 35 ± 2°C for 24 - 48 hours. Since many pathogens require  arbon dioxide on primary isolation, plates may be incubated in an atmosphere containing approximately 5 - 10% CO2. Results:
1. Alpha-hemolysis: greenish discoloration of medium
2. Beta-hemolysis: clear zone surrounding colony
3. Gamma-hemolysis: no change

MICROBIOLOGICAL TEST
The following results were obtained in the performance of the medium from type cultures, with 5% of defi brinated sheep blood, after incubation at emperature of 35 ± 2°C and observed after 24 - 48 hours.