Prepared Plated Media PPM

BRAIN HEART INFUSION AGAR

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Catalog Number : B02

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Description

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BRAIN HEART INFUSION AGAR is used for the cultivation of fastidious bacteria (streptococci, pnemococci, meningococci, etc.) and is also recommended for the cultivation of pathogenic fungi. Collect, dilute and prepare samples and volumes as required according to specifications, directives, official standard regulations and/or expected results. Spread the plates by streaking methodology or by spiral method. Incubate the plates right side up aerobically at 37+/-1ºC for 24-48h.

(Incubation times greater then those mentioned above or different incubation temperatures may be required dpending on the sample, on the specifications,... This medium can be inoculated directly or after enrichment broth). After incubation, enumerate all the colonies that have appeared onto the surface of the agar. Each laboratory must evaluate the results according to their specifications. Calculate total microbial count per ml of sample by multiplying the average number of colonies per plate by the inverse dilution factor if streaked a diluted sample. Report results as Colony Forming Unit (CFU’s) per ml or g along with incubation time and temperature.