Prepared Plated Media PPM

BRAIN HEART INFUSION BROTH

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Catalog Number : B01

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Description

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BRAIN HEART INFUSION BROTH (BHIB) is a liquid medium rich in nutrients, suitable for the cultivation of several fastidious strains of bacteria, such as streptococci, meningococci and pneumococci, fungi and yeasts. BHIB is recommended in Standard Methods for water testing and in antimicrobial susceptibility tests. Tubes of 0.5 ml BHI broth are used to cultivate bacteria used in the preparation of inocula for use in microdilution minimal inhibitory concentration (MIC) and identifi cation (ID) test panels. The nutritionally rich base of Beef heart and Calf brain infusions and Peptone mixture provide nitrogen, vitamins, minerals and amino acids essential for growth of a variety of microorganisms. Dextrose is the carbon energy source and Sodium chloride maintains the osmotic balance. This medium is very versatile and supports the growth of many fastidious organisms. With the addition of 0.1% agar, the medium is used for the cultivation of anaerobes. Adding 0.1% agar reduces the fl ow of oxygen convection currents and encourages the development of anaerobes and microorganisms. BHI Broth is recommended for the preparation of the culture of S.aureus for use in coagulase tests. Inoculate and incubate at 35 ± 2°C for 18 - 24 hours.

MICROBIOLOGICAL TEST
The following results were obtained in the performance of the medium from type cultures after incubation at a temperature of 35 ± 2°C and observed after 18 - 24 hours.