Prepared Plated Media PPM

CLED AGAR

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Catalog Number : 914

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Description

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CLED AGAR is a non-selective diff erential plating medium for the growth and enumeration of urinary tract microorganisms. Omitting sodium chloride inhibits the Proteus swarming and supports the growth of the vast majority of bacteria causing urinary tract infections, and is used to diff erentiate and identify them. The presence of bacterial contaminants like Diphtheroids, lactobacilli and other microbes indicate the degree of care taken with the handling of the urine specimen.Beef Extract and Casein peptone provide nitrogen, vitamins, minerals and amino acids essential for growth. Lactose is the fer mentable carbohydrate providing carbon and energy. L-Cystine is added as a growth supplement for cystine dependent coli forms. Diff erentiation of lactose fermenters and lactose non fer menters is achieved using Bromothymol blue as a pH indicator. Organisms that ferment lactose will lower the pH and change the color of the medium from green to yellow. Bacteriological agar is the solidifying agent.The microorganisms which cause infection in the urinary tract are generally abundant and of only one species. E. coli is the or ganism most frequently isolated. The seeding of the sample can be done by the dilution method or by streaking on the surface of agar with a calibrated loop. Count the colonies after 24 - 48 hours of incubation at a temperature of 35 ± 2°C. Report the number of colonies per ml of urine. A count of 100.000 (105)/ml or more is an indication of a signifi cant clinical urinary tract infection.

MICROBIOLOGICAL TEST
The following results were obtained in the performance of the medium from type cultures after incubation at a temperature of 35 ± 2ºC and observed after 24 - 48 hours.