Prepared Plated Media PPM

SABOURAUD DEXTROSE AGAR

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Catalog Number : 907

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Description

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SABOURAUD DEXTROSE AGAR can be used for cultivating yeasts, molds and aciduric microorganisms. It is used for cultivating pathogenic fungi, particularly those associated with skin infections. This medium is also used for determining the microbial and fungal content of cosmetics and for the mycological evaluation of food. The formula is based on the European Pharmacopoeia. Dextrose is the fermentable carbohydrate providing carbon and energy. Peptone mixture provides nitrogen, vitamins, minerals and amino acids essential for growth. Bacteriological agar is the solidifying agent. The high dextrose concentration and acidic pH make this medium selective for fungi.Georg et al demonstrated that the basic agar fortifi ed by three antibiotics considerably improves the isolation of pathogenic fungi from heavily contaminated sources. To prepare a selective culture medium aseptically add the following antibiotics: Cycloheximide, Penicillin and Streptomycin.The incubation of the plates should be at 30ºC for 3 - 7 days.The European Pharmacopoeia recommends this medium in the Paragraph 2.6.12 Microbiological examination of non- sterile products: Microbial enumeration test. For promoting the growth of Candida albicans ATCC 10231 and Aspergillus brasiliensis ATCC 16404 to inoculate ≤ 100 CFU at 20 - 25ºC for ≤ 5 days.

The European Pharmacopoeia recommends in the Paragraph 2.6.13 “Microbiological examination of non-sterile products” Test for specifi ed microorganisms Use Dextrose Sabouraud Agar for growth promotion. After incubation at 30 - 35ºC for 3 - 5 days in Dextrose Sabouraud Broth subculture on the plate of Dextrose Sabouraud Agar and incubate at 30 - 35ºC for 24 - 48 hours.Interpretation Growth of white colonies may indicate the presence of Candida albicans. This is confi rmed by identifi cation tests.The product complies with the test if such colonies are not present or if the confi rmatory identifi cation tests are negative.

MICROBIOLOGICAL TEST
The following results were obtained in the performance of the medium from type cultures after incubation at a temperature of 30ºC and observed after 3 - 7 days.