Prepared Plated Media PPM

SALMONELLA SHIGELLA AGAR

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Catalog Number : 909

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Description

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SALMONELLA SHIGELLA AGAR (SS AGAR) is a selective and diff erential medium widely used in sanitary bacteriology to isolate Salmonella and Shigella from feces, urine, and fresh and canned foods. Due to its strong inhibitory power, SS Agar can be streaked with a heavy inoculum, but other less inhibitory media, such as Desoxycholate Agar (Cat. 1020), MacConkey Agar (Cat. 1052), Eosin Methylene Blue (EMB) Agar (Cat. 1039), XLD Agar (Cat. 1080) and Hektoen Enteric Agar (Cat. 1030), should be streaked in parallel. Inoculate and incubate at 35 ± 2°C for 18 - 24 hours. Beef extract and Peptone mixture provide nitrogen, vitamins, minerals and amino acids essential for growth. Lactose is the fermentable carbohydrate providing carbon and energy. Bile Salts Mixture, Sodium citrate and Brilliant green inhibit Gram positive bacteria, most coliform bacteria and swarming Proteus spp., while allowing Salmonella spp. to grow. Neutral red is the pH indicator. Sodium thiosulfate and Ferric citrate allow the detection of the H2S producing bacteria, such as Proteus and some strains of Salmonella, since they produce colonies with black centers and a clear halo. Non-lactose fermenting bacteria (supposed pathogens) produce clear colonies, transparent or colorless, while coliforms are suffi ciently inhibited, and form small colonies that vary from pink to red in color. The plates of the medium can be kept for at least a week in refrigeration. This formulation, highly selective, is not recommended for the primary isolation of Shigella. Some Shigella spp. may be inhibited.

MICROBIOLOGICAL TEST
The following results were obtained in the performance of the medium from type cultures after anaerobic incubation at a temperature of 35 ± 2°C and observed after 18 - 24 hours.